High–Fidelity DNA Polymerase, blunt end cloning is recommended. If TA cloning is required, it can be performed by adding A overhangs to the blunt PCR product with Thermo Scientific ™ Taq. DNA Polymerase (#EP), for example. Incubate purified PCR product with 1x. Taq. buffer, mM MgCl. 2, mM dATP and 1 U. Taq. DNA polymerase in. · It s perfect for amplifying short DNA targets (up to 2 kb), regardless of GC content or template purity. Terra PCR Direct is a highly sensitive enzyme that allows. (PR; published September ) Terra™ PCR Direct Polymerase Mix Protocol-at-a-Glance (PT) Please read the User Manual before using this Protocol-at-a-Glance. BD Advantage™ 2 PCR Kits Protocol-at-a-Glance (PT) Please read the User Manual before using this abbreviated protocol. The Protocol-at-a-Glance is provided for your con-venience, but is not intended for first-time users. (PR) New Product Size Cat. No. Cat. No. BD Advantage™ 2 Polymerase Mix rxns rxns
50X polymerase mix containing a combination of DNA polymerases suitable for long-distance PCR. We recommend ®Clontech’s Advantage 2 Polymerase Mix (Cat. Nos. , ), which was specificallydeveloped for LD. User Manual Cat. No. PT (PR) Published 27 August protocol are optimized for use with the Advantage® 2 Polymerase Mix. BD Advantage™ 2 PCR Kits Protocol-at-a-Glance (PT) Please read the User Manual before using this abbreviated protocol. The Protocol-at-a-Glance is provided for your con-venience, but is not intended for first-time users. (PR) New Product Size Cat. No. Cat. No. BD Advantage™ 2 Polymerase Mix rxns rxns
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