Advantage gc 2 pcr user manual

BD Advantage™ 2 PCR Kits Protocol-at-a-Glance (PT) Please read the User Manual before using this abbreviated protocol. The Protocol-at-a-Glance is provided for your con-venience, but is not intended for first-time users. BD Advantage™ GC 2 PCR Kit 10 rxns Ky 7 rows · Advantage® GC 2 PCR Kit. 10 Rxns. USD $ This kit is designed for Missing: user manual. The Advantage GC 2 PCR Kit (Cat. Nos. ) includes Advantage GC 2 Polymerase Mix, our proprietary GC-Melt Reagent, and a reaction buff er containing DMSO. This kit allows the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques, including sequences that are up to 90% GC, with .

The Advantage GC 2 PCR Kit (Cat. Nos. ) includes Advantage GC 2 Polymerase Mix, our proprietary GC-Melt Reagent, and a reaction buff er containing DMSO. This kit allows the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques, including sequences that are up to 90% GC, with minimal buffer optimization. Advantage® GC 2 PCR Kit. 10 Rxns. USD $ This kit is designed for efficient and accurate amplification of GC-rich (up to 90%) templates. It is based on the hot-start polymerase Advantage 2 polymerase mix. This kit contains individual tubes of Advantage-GC 2 Polymerase Mix, special GC-buffers, and dNTPs. Advantage®-HF 2 PCR User Manual I. Introduction continued For amplifying GC-rich genomic templates, use our Advantage-GC Genomic Kit (Cat. Nos. , ). These kits are recommended for use with Clontech's GenomeWalker Kits (Cat. Nos. , , , , and several LD-Insert Screening Amplimer Sets.

The result demonstrated the superiority of the 2-step PCR protocol over to the manufacturer's instructions for Gram-positive bacteria. Digital PCR (dPCR) uses the procedure of end-point PCR but splits the PCR reaction into many single partitions, in which the template is randomly. iProof™ High Fidelity DNA. Polymerase, 2 units/µl. iProof HF Buffer. ml. 3 x ml. 5X HF Buffer, mM MgCl2. iProof GC Buffer. ml. 3 x ml.